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KMID : 0379519990150030363
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한국독성학회지
1999 Volume.15 No. 3 p.363 ~ p.367
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Effect of Cordyceps militaris on the Mutagenicity and DNA Fragmentation of E. coli
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Lee Wan-Hee
Kwon Hyeok-Yil
Park Jin-Seu
Choi Soo-Young
Lee Kil-Soo
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Abstract
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Cordyceps militaris is one of the parasitic fungi Cordyceps sp. and used for Chinese traditional herbal medicine. Some components of Cordyceps sinensis extract such as cordycepin, certain polysaccharides and even it’s crude extract are significantly effective on the immune system, enzyme inhibition, reveal anticancer and antifungal activity in animal experimental system. Protecting activity of Cordyceps militaris extract (CME) against DNA damage and antimutagenic activity of CME were investigated in microbial test system and in DNA fragmentation experiment. CME inhibited not only chromosomal DNA damage in E. coli, but also daunorubicin-induced plasmid DNA damage. It was observed that DNA damage inhibition in vitro by CME resulted from the inactivation of daunorubicin by CME, but not from cellular DNA protection by CME. In vivo mutagenicity test system in E. coli, CME did not repair DNA damage induced by 4-nitroquinoline-n-oxide (4NQO), but it caused the 4NQO inactivation which leads to inhibit 4NQO-induced β-galactosidase synthesis in SOS-chromotest. From the correlation between the inhibition of in vitro DNA fragmentation with the inhibition of cellular DNA damage in vivo, it is suggested that CME has an inhibitory effect on the DNA damaging action of daunorubicin and on the mutagenicity of 4NQO in E. coli test system.
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KEYWORD
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Cordyceps militaris, DNA damage, DNA fragmentation, Antimutagenic action, SOS-chro-motest
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